Journal: Pharmaceutics

Article Title: Olaparib Conjugates with Selenopheno[3,2- c ]quinolinone Inhibit PARP1 and Reverse ABCB1-Related Multidrug Resistance

doi: 10.3390/pharmaceutics14122571

Figure Lengend Snippet: Cytotoxicity of 5a – c on the MES-SA/Dx-5 cell line.

Article Snippet: MES-SA (Human uterine sarcoma, ATCC ® CRL-1976TM) and H9C2 (rat cardiomyocytes, ATCC CRL-1446TM), MCF-7 (human adenocarcinoma, ATCC HTB-22TM), and HCC1937 (human breast carcinoma, ATCC CRL-2336TM) cell lines were obtained from American Type Culture Collection, and the MES-SA/Dx-5 (doxorubicin resistant human uterine sarcoma with high levels of MDR1 mRNA and P-glycoprotein, ECACC 95051031-1VL) cell line was obtained from the European Collection of Authenticated Cell Cultures.

Techniques:

Journal: Journal of the National Cancer Institute

Article Title: Restoring Anticancer Immune Response by Targeting Tumor-Derived Exosomes With a HSP70 Peptide Aptamer.

doi: 10.1093/jnci/djv330

Figure Lengend Snippet: Figure 2. Exosomes derived from human and mouse cancer cell lines express HSP70 on their membranes. A) A representative western blot of Flotillin-1 and CD81 expression in exosomes isolated from mouse embryonic fibroblasts (MEFs), B16F10, CT26, HCT116, and SW480 cells’ supernatants (n = 3). B) Membrane-bound HSP70 in exosomes isolated from B16F10, CT26, HCT116, SW480, MEF, and normal colon mucosa (NCM) was determined by flow cytometry. Data represent means ± SD of the ratio of MFI cmHSP70/ MFI Ig control (n = 3). Two-sided t test: *P < .05. Lower panels are representative FACS histograms. C) Binding of exosomes (nm), derived from MEF, NCM, B16F10, CT26, HCT116, and SW480 cells to immobilized biotinylated A8 was determined by biolayer interferometry. MEF and NCM that showed little bind- ing are considered negative controls for HSP70 exosomes. Binding curves represent mean signal of triplicate measurements. Mann-Whitney: ***P < .001. D) Binding of exosomes (nm), derived from PC3, HeLa, MCF-7, and EL4 cancer cells, to immobilized biotinylated A8 was determined by biolayer interferometry. Each cancer cell was compared with the following normal counterparts: prostate epithelial cells (PrEC), primary uterine cells (PUC), human mammary epithelial cells (HMECs), and primary lymphocytes (PLs). Mann-Whitney: ***P < .001. All statistical tests were two-sided. Binding curves represent mean signal of triplicate measurements.

Article Snippet: B16F10 (mouse melanoma), CT26 (mouse colon cancer), HCT116 (human colon cancer), SW480 (human colon cancer), PC3 (human prostate cancer), HeLa (human cervix cancer) EL4 (mouse lymphoma) cell lines (American Type Culture Collection [ATCC]) were cultured in RPMI 10% foetal bovine serum (FBS; Lonza); mouse embryonic fibroblasts (MEF) were cultured in DMEM 10% FBS (Lonza).

Techniques: Derivative Assay, Western Blot, Expressing, Isolation, Membrane, Flow Cytometry, Control, Binding Assay, MANN-WHITNEY